Double antibody enzyme immunoassay for the quantitation of adenosine 3', 5' -cyclic monophosphate (cyclic AMP) and guanosine 3', 5'-cyclic monophosphate (cyclic GMP) in tissue and plasma.
作者： I. Yamamoto , J. Tsuji , T. Takai , M. Fujimoto
DOI: 10.1080/15321818208056994
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摘要: Abstract A sensitive double antibody enzyme immunoassay for the quantitation of cyclic AMP and GMP is presented. Specific antisera to each nucleotide were raised in rabbits by immunization with succinyl nucleotide-human serum albumin conjugates. For competitive reaction, antibodies incubated a mixture labelled β-D-galactosidase unlabelled succinylated standard or sample nucleotides. The antibody-bound enzyme-hapten was separated from free hapten anti-rabbit IgG immobilized polystyrene ball. Activity on solid phase fluorometrically determined. assay system made it possible ascertain values as low 5 fmole GMP. Cyclic nucleotides plasma could be accurately determined this method without requiring deproteinizing reagent first step assay.
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