Development of a sensitive enzyme immunoassay for OPC-7251, a novel antimicrobial agent for percutaneous application.

摘要: A sensitive enzyme immunoassay for OPC-7251, a novel pyridone carboxylic acid antimicrobial agent, was developed and applied the determination of human plasma levels. OPC-7251 coupled to bovine serum albumin through formation N-hydroxysuccinimide ester. By immunization rabbits, highly specific antiserum raised. Using beta-D-galactosidase-labeled hapten, homologous assay system allowed detection 2 pg this compound. Plasma samples were precisely analyzed down minimum value 200 pg/ml after heat treatment. The further validated by recovery test correlation with HPLC analyses. Percutaneous application 10 g 1% cream healthy volunteers resulted in peak 1.6 ng/ml about 8 hours dosing, indicating extremely low absorption efficiency transdermal system.