Efficient In Vitro Micropropagation and Regeneration of Humulus Lupulus on Low Sugar, Starch-Gelrite Media
作者： I. Smykalova , M. Ortova , H. Lipavska , J. Patzak
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摘要: Several Czech and foreign hop mericlones were tested in vitro for efficiency of green callus formation plant regeneration from internodal or nodal explants. Modified MS media gelled either with agar, starch a mixture potato Gelrite, supplemented different concentrations glucose maltose, investigated. Two (Osvald 72 no. 5216 Sladek 6908) studied more details because their capacities. The HPLC analysis medium sugar after the explant cultivation has revealed slow uptake medium. Presence at concentration 45 g dm−3 agar resulted decreased number nodes compared to control 30 glucose. use plus Gelrite instead routinely used decreasing 15 proved be most efficient multiplication rate. this results lower cost micropropagation healthy cultures without exhibition vitrification.
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Rose Galzy, Daniel Compan, Remarks on mixotrophic and autotrophic carbon nutrition of Vitis plantlets cultured in vitro Plant Cell Tissue and Organ Culture. ,vol. 31, pp. 239- 244 ,(1992) , 10.1007/BF00036230
Margareta Welander, Nathalie Pawlicki, Carbon compounds and their influence on in vitro growth and organogenesis Springer, Dordrecht. pp. 83- 93 ,(1994) , 10.1007/978-94-011-0790-7_9
Richard H. Zimmerman, Ingrid M. Fordham, S. V. Bhardwaj, Use of starch-gelled medium for tissue culture of some fruit crops Plant Cell Tissue and Organ Culture. ,vol. 43, pp. 207- 213 ,(1995) , 10.1007/BF00039946
M.T.S. Trevisan, J.J.C. Scheffer, R. Verpoorte, Effect of elicitation on the peroxidase activity in some cell suspension cultures of hop, t Humulus lupulus Plant Cell Tissue and Organ Culture. ,vol. 48, pp. 121- 126 ,(1997) , 10.1023/A:1005824927070
Paul D. Sherrington, Edwin F. George, Plant propagation by tissue culture : handbook and directory of commercial laboratories Exegetics. ,(1984)
Kenneth S. Kang, George T. Veeder, Peter J. Mirrasoul, Tatsuo Kaneko, Ian W. Cottrell, Agar-Like Polysaccharide Produced by a Pseudomonas Species: Production and Basic Properties Applied and Environmental Microbiology. ,vol. 43, pp. 1086- 1091 ,(1982) , 10.1128/AEM.43.5.1086-1091.1982
Use of various carbon sources by isolated maple callus cultures Plant and Cell Physiology. ,vol. 14, pp. 797- 801 ,(1973) , 10.1093/OXFORDJOURNALS.PCP.A074916
S. Rakousky, J. Matousek, Direct organogenesis in hop - a prerequisite for an application ofA. tumefaciens-mediated transformation Biologia Plantarum. ,vol. 36, pp. 191- 200 ,(1994) , 10.1007/BF02921085
W. E. Henderson, A. M. Kinnersley, Corn starch as an alternative gelling agent for plant tissue culture Plant Cell Tissue and Organ Culture. ,vol. 15, pp. 17- 22 ,(1988) , 10.1007/BF00039885
M. J. PAUL, M. STITT, Effects of nitrogen and phosphorus deficiencies on levels of carbohydrates, respiratory enzymes and metabolites in seedlings of tobacco and their response to exogenous sucrose Plant Cell and Environment. ,vol. 16, pp. 1047- 1057 ,(1993) , 10.1111/J.1365-3040.1996.TB02062.X