作者: Kenneth S. Kang , George T. Veeder , Peter J. Mirrasoul , Tatsuo Kaneko , Ian W. Cottrell
DOI: 10.1128/AEM.43.5.1086-1091.1982
关键词: Biochemistry 、 Incubation 、 Chemistry 、 Pseudomonas 、 Polysaccharide 、 Melting point 、 Enzyme 、 Uronic acid 、 Agar 、 Chromatography 、 Rhamnose 、 Biotechnology 、 Food science 、 Ecology (disciplines) 、 Applied Microbiology and Biotechnology
摘要: A new species of Pseudomonas was isolated that produced copious amounts an exocellular heteropolysaccharide (PS-60) after incubation for 3 days at 30°C in media containing 3% glucose as a carbon source. The polysaccharide composed approximately 46% and 30% rhamnose and, addition, contained 21% uronic acid O-acetyl. Upon deacetylation by mild alkaline treatment, PS-60 brittle, firm, optically clear gel. This gelling property thermoreversible. gel exhibited excellent heat stability withstood autoclaving (i.e., 121°C 15 min) several cycles. strength, melting point, setting point the were controlled primarily concentration cations. not affected variety enzymes. results tests involving various culture biochemical test indicate is alternative agent to agar.