Agar-Like Polysaccharide Produced by a Pseudomonas Species: Production and Basic Properties

作者: Kenneth S. Kang , George T. Veeder , Peter J. Mirrasoul , Tatsuo Kaneko , Ian W. Cottrell

DOI: 10.1128/AEM.43.5.1086-1091.1982

关键词: BiochemistryIncubationChemistryPseudomonasPolysaccharideMelting pointEnzymeUronic acidAgarChromatographyRhamnoseBiotechnologyFood scienceEcology (disciplines)Applied Microbiology and Biotechnology

摘要: A new species of Pseudomonas was isolated that produced copious amounts an exocellular heteropolysaccharide (PS-60) after incubation for 3 days at 30°C in media containing 3% glucose as a carbon source. The polysaccharide composed approximately 46% and 30% rhamnose and, addition, contained 21% uronic acid O-acetyl. Upon deacetylation by mild alkaline treatment, PS-60 brittle, firm, optically clear gel. This gelling property thermoreversible. gel exhibited excellent heat stability withstood autoclaving (i.e., 121°C 15 min) several cycles. strength, melting point, setting point the were controlled primarily concentration cations. not affected variety enzymes. results tests involving various culture biochemical test indicate is alternative agent to agar.

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