Reattachment of retinas to cultured pigment epithelial monolayers from Xenopus laevis.
作者： K C Easterling , D M Defoe
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摘要: PURPOSE The authors aimed to establish an in vitro model of the retinal pigment epithelium (RPE) suitable for studies neural retina-epithelium interactions. METHODS Sheets RPE were obtained from Xenopus laevis eyes by Dispase treatment intact globes. After dispersal trypsin-EDTA solution, cells plated onto Matrigel-coated microporous membrane filters and grown a serum-free defined medium. RESULTS Confluent cell monolayers after 7 10 days culture appeared electron microscopy be morphologically polarized, established junctional complexes, exhibited transepithelial resistances ranging 300 500 omega.cm2. As did native epithelium, these formed cytoskeletons consisting circumferential bands actin myofilaments at their periphery whorls cytokeratin intermediate filaments throughout cytoplasm. Co-culture freshly isolated retinas with resulted apparent reattachment photoreceptors within 3 hours. CONCLUSIONS Primary cultures amphibian that express phenotypic characteristics situ are also capable establishing adhesive interactions retinas.
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