Critical role of TrkB and brain-derived neurotrophic factor in the differentiation and survival of retinal pigment epithelium
作者： Zheng Z. Liu , Ling Q. Zhu , Fernette F. Eide
DOI: 10.1523/JNEUROSCI.17-22-08749.1997
关键词:
摘要: In the vertebrate eye, retinal pigment epithelium (RPE) and neural retina arise from a single layer of neuroectoderm. Factors influencing differentiation neurons have been identified; however, little is known about molecules directing RPE. Here we found that neurotrophin brain-derived neurotrophic factor (BDNF) plays an autocrine role in survival Xenopus laevis Fluorescent situ hybridization studies showed precise co-expression BDNF its receptor trkB neuroepithelium actively differentiating RPE; vitro demonstrated survival- differentiation-promoting effects serum-free explants dissociated cultures. When dominant negative mutant was expressed developing embryos, severe arrest RPE seen with persistence nestin- Notch-positive neuroblasts.
sci-hub.se 参考文章(51)
J. Pérez, E. Perentes, Light-induced retinopathy in the albino rat in long-term studies: An immunohistochemical and quantitative approach Experimental and Toxicologic Pathology. ,vol. 46, pp. 229- 235 ,(1994) , 10.1016/S0940-2993(11)80088-6
K C Easterling, D M Defoe, Reattachment of retinas to cultured pigment epithelial monolayers from Xenopus laevis. Investigative Ophthalmology & Visual Science. ,vol. 35, pp. 2466- 2476 ,(1994)
I. Imafuku, I. Kanazawa, H. Okazawa, Mm. Kamei, Gene regulation of trkB and trkC in the chick retina by light/darkness exposure. Oncogene. ,vol. 9, pp. 1813- 1818 ,(1994)
Jane A. Langdale, In situ Hybridization Springer, New York, NY. pp. 165- 180 ,(1994) , 10.1007/978-1-4612-2694-9_18
Peter D. Vize, Douglas A. Melton, Ali Hemmati-Brivanlou, Richard M. Harland, Chapter 20 Assays for Gene Function in Developing Xenopus Embryos Methods in Cell Biology. ,vol. 36, pp. 367- 387 ,(1991) , 10.1016/S0091-679X(08)60288-5
Michael W. Klymkowsky, James Hanken, Whole-mount staining of Xenopus and other vertebrates. Methods in Cell Biology. ,vol. 36, pp. 419- 441 ,(1991) , 10.1016/S0091-679X(08)60290-3
Takashi Tohyama, VM-Y Lee, LB Rorke, M Marvin, RD McKay, JQ Trojanowski, None, Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Laboratory Investigation. ,vol. 66, pp. 303- 313 ,(1992)
Paola Bovolenta, José-Marı́a Frade, Elisa Martı́, Marı́a-Angeles Rodrı́guez-Peña, Yves-Alain Barde, Alfredo Rodrı́guez-Tébar, Neurotrophin-3 Antibodies Disrupt the Normal Development of the Chick Retina The Journal of Neuroscience. ,vol. 16, pp. 4402- 4410 ,(1996) , 10.1523/JNEUROSCI.16-14-04402.1996
EF Wheeler, M Bothwell, Spatiotemporal patterns of expression of NGF and the low-affinity NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogenesis The Journal of Neuroscience. ,vol. 12, pp. 930- 945 ,(1992) , 10.1523/JNEUROSCI.12-03-00930.1992
G I Evan, G K Lewis, G Ramsay, J M Bishop, Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Molecular and Cellular Biology. ,vol. 5, pp. 3610- 3616 ,(1985) , 10.1128/MCB.5.12.3610