Isolation of Rare Cells by High-Speed Flow Cytometry and High-Resolution Cell Sorting for Subsequent Molecular Characterization - Applications in Prenatal Diagnosis, Breast Cancer and Autologous Bone Marrow Transplantation

摘要: As we have discussed previously (1), analysis and isolation of rare cell subpopulations been interest to researchers clinicians in many areas biology medicine including: a) detection somatic mutations mutagenized cells (2), b) human fetal maternal blood for prenatal diagnosis birth defects (3), c) CALLA+ (4), d) minimal residual diseases (5,6), e) stem (7), f) HIV-infected peripheral (8). Unfortunately, conventional flow cytometer/cell sorters operating at rates below 10,000 cells/sec require hours analyze and/or isolate low frequencies (e.g. 10-4 – 10-7) making them impractical use routine sorting such subpopulations. One simple method processing higher speeds on cytometers has described (9). This triggers the data acquisition or sort signal a fluorescence signal.