In-depth analysis of molecular alterations within normal and tumour tissue from an entire bronchial tree.

摘要: Using laser capture microdissection (LCM), fluorescent microsatellite analysis and immunohistochemical analysis, we have constructed a detailed topographical molecular map of the entire bronchial tree surrounding primary squamous carcinoma in order to establish relationship between damage within airway that tumour itself. Allelic imbalance was analysed using markers on chromosomes 3, 9, 13 17. In addition, for p53 cyclin D1 expression performed. Analysis revealed allelic at several loci site but also 83% histologically normal specimens upper lower lobes. The fractional allele loss (FAL) value statistically higher (0.75+/-0.13) than distal (0.42+/-0.09) lobes (0.31+/-0.08). Immunohistochemical overexpression protein epithelium, thus confirming previous reports their early involvement lung development. This is date largest in-depth study LCM single individual. patterns allele-specific observed support clonal or oligoclonal expansion model outgrowths throughout lung. widespread incidence genetic changes whole most likely represents smoking-induced alterations emphasize complexity field cancerization concept. Our findings point need studies tissue carcinomas, identify differentiate preneoplastic neoplastic stages carcinogenesis.