Elevation of the intracellular pH activates respiration and motility of sperm of the sea urchin, Strongylocentrotus purpuratus.
作者： R Christen , R W Schackmann , B M Shapiro
DOI: 10.1016/S0021-9258(18)33365-9
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摘要: The internal pH (pHi) of sperm the sea urchin, Strongylocentrotus purpuratus, was estimated by measuring accumulation weak bases [14C]methylamine, [14C]diethylamine, or 9-aminoacridine under conditions where cellular respiration activated inhibited. When fluorescence measurements are corrected for binding to intracellular components, pHi estimates agree quantitatively with those obtained from [14C]amine distributions. decreased when [K+] elevated above physiological value 10 mM, external substantially below 8.0, Na+ absent seawater. At values, and motility were inhibited; conversely, both increased elevated. Increased occurred whether altering pH, [Na+], addition NH4Cl medium. In all cases, activation linked, suggesting a unitary control mechanism, some possibilities which presented.
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