Accumulation of DNA methylation alterations in paediatric glioma stem cells following fractionated dose irradiation.

摘要: Radiation is an important therapeutic tool. However, radiotherapy has the potential to promote co-evolution of genetic and epigenetic changes that can drive tumour heterogeneity, formation radioresistant cells relapse. There a clinical need for better understanding DNA methylation alterations may follow be able prevent development radiation-resistant cells. We examined radiation-induced in profiles paediatric glioma stem (GSCs) vitro. Five GSC cultures were irradiated vitro with repeated doses 2 or 4 Gy. was given 3 15 fractions. profiling using Illumina arrays performed at 14 days post-radiation. The cellular characteristics studied parallel. Few fractions radiation did not result significant accumulation alterations. extended dose fractionations changed induced thousands differentially methylated positions, specifically enhancer regions, sites involved alternative splicing repetitive regions. dose-dependent morphological proliferative as consequence exposure. regulatory functions proliferation differentiation identified, which reflect response stress through reprogramming cues.