The tissue plasminogen activator gene promoter: a novel tool for radiogenic gene therapy of the prostate?

摘要: Background Radiation therapy is a treatment modality routinely used in cancer management so it not unexpected that radiation-inducible promoters have emerged as an attractive tool for controlled gene therapy. The human tissue plasminogen activator promoter (t-PA) has been proposed candidate radiogenic therapy, but exploited to date. purpose of this study was evaluate the potential drive expression reporter gene, green fluorescent protein (GFP), response radiation exposure. Methods To investigate whether could be prostate we initially transfected normal and malignant cells. We then HMEC-1 endothelial cells ex vivo rat tail artery monitored GFP levels using Western blotting following delivery single doses ionizing (2, 4, 6 Gy) test vascular targeted Results t-PA induced up 6-fold all cell types tested within clinical range. Conclusions These results suggest may incorporated into strategies driving therapeutic transgenes conjunction with Copyright (C) 2008 John Wiley & Sons, Ltd.