Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin.
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摘要: In order to characterize the glycoconjugate residues in skin and gills of adult rainbow trout, binding pattern five biotinylated lectins with different carbohydrate specificities was examined. skin, mucous cells revealed sites for PNA SBA; filament-containing were additionally labelled Con A. However, basal cell layer showed no reaction. gill, subpopulations reacted A, PNA, SBA UEA-I. This broader spectrum glycoconjugates gill compared epidermal could point additional function mucus ion osmoregulation. Lectin less common respiratory epithelial secondary lamellae than those primary lamellae. Chloride mannose, galactose fucose residues. Immature chloride cells, as indicated by a comparison Na+/K+ ATPase immunolabelling, A; them The results form basis further investigations which these populations can be analysed under environmental conditions
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