Transcriptional profiling of human gingival fibroblasts in response to multi-species in vitro subgingival biofilms.

摘要: Summary Periodontitis is an infectious inflammatory disease that destroys the tooth-supporting tissues. It initiated by complex subgingival biofilms, triggering response juxtaposed gingival tissue. The range of transcriptional events in gingiva following biofilm challenge not fully elucidated. By employing gene microarray technology, this study aimed to characterize overall changes (more than two-fold regulation) cultured human fibroblasts a 10-species vitro model (BF), over period 6 h. relative involvement three ‘red complex’ species these was evaluated omitting from composition (BF-RC). When compared with unchallenged control, BF and BF-RC differentially regulated 386 428 genes, respectively, overlap 52–75%. Interestingly, expression only genes significantly different between challenged groups. There also strong affected signalling pathways ontology processes. These involved primarily immune response, included toll-like receptors, interleukin-1, interleukin-17 heat-shock proteins 60 70. In conclusion, biofilms elicited large number fibroblasts, while presence did yield any substantial differences. findings show uniform ‘non-specific’ host cells denote redundancies may exist virulence properties individual bacterial within polymicrobial community.