Methods for preparing conjugates

摘要: Methods are disclosed for conjugating one moiety to another moiety. In the method moieties reacted with in a protic solvent. Reaction between and solvent during is negligible or reversible. A stable bond formed produce product that not subject β-elimination at elevated pH. Usually, of comprises an unsaturation two carbon atoms. One atoms becomes electrophile conjugating. The other functionality reactive atom form unsaturation. Compounds comprising both as well precursor molecules also disclosed. determining analyte sample employing compounds described above.