Effect of human activated NRAS on replication of delNS1 H5N1 influenza virus in MDCK cells
作者： Jiping Zhu , HongBo Zhou , Wei Zou , MeiLin Jin
关键词:
摘要: Background: RAS, coded by ras proto-oncogenes, played an important role in signal transmission to regulate cell growth and differentiation. Host activation of RAS was significant for IFN-sensitive vaccinia virus (delE3L) or attenuate influenza unallowable cells. Results: Huamn NRAS gene activated mutating codon 61. Then the detected western blot MDCK The delNS1 H5N1 with deletion NS1 eIF4GI binding domain weak multiplication And replication expression IFN-beta IRF-3 were Real-time PCR cells infected virus. It found that had a increase when activated, yet, restrained. Conclusions: study demonstrated part Activated might be down-regulating antiviral cellular factors
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