Intestinal Inflammation Increases the Expression of the P2Y6 Receptor on Epithelial Cells and the Release of CXC Chemokine Ligand 8 by UDP
作者： Djordje M. Grbic , Émilie Degagné , Christine Langlois , Andrée-Anne Dupuis , Fernand-Pierre Gendron
DOI: 10.4049/JIMMUNOL.180.4.2659
关键词:
摘要: Epithelial cells participate in the immune response of intestinal mucosa. Extracellular nucleotides have been recognized as inflammatory molecules. We investigated role extracellular and their associated P2Y receptors secretion cytokines by epithelial cells. The effect inflammation on P2Y6 receptor expression was determined PCR mouse, rat, human. Localization immunofluorescence microscopy colon normal dextran sulfate sodium-treated mice. activation UDP cytokine release using a combination Western blots, luciferase assays, RT-PCR, Ab arrays, ELISA. Inflammation up-regulates P2Y2 well mucosa colitic In vitro, we demonstrated that could be released Caco-2/15 confirmed increased challenging cell-6 with TNF-α IFN-γ showing stimulation results an CXCL8 ERK1/2-dependent mechanism. increase transcriptional receptor. This study is first report demonstrating implication show for time P2Y6, P2Y2, stress inflammation. These demonstrate emergence nucleotide signaling orchestration
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