Establishment of Protocols for Global Metabolomics by LC-MS for Biomarker Discovery

摘要: Metabolomics is a promising avenue for biomarker discovery. Although the quality of metabolomic analyses, especially global metabolomics (G-Met) using mass spectrometry (MS), largely depends on instrumentation, potential bottlenecks still exist at several basic levels in workflow. Therefore, we established precise protocol initially G-Met analyses human blood plasma to overcome some these difficulties. In our protocol, samples are deproteinized 96-well plate an automated liquid-handling system, and conducted either UHPLC-QTOF/MS system equipped with reverse phase column or LC-FTMS normal column. A normalization data was also developed compensate intra- inter-batch differences, variations were significantly reduced along normalization, C18 reverse-phase positive ions. Secondly, examined changes profiles caused by storage EDTA-blood specimens identify markers evaluation specimens’ pre-analytical conditions. Forty markers, including lysophospholipids, dipeptides, fatty acids, succinic acid, amino glucose, uric acid identified sample equation calculating score. We applied small-scale study evaluate clinical samples. The protocols here should prove useful discovery development biomarkers wider range diseases.