Direct quantification of unencapsulated doxorubicin in liposomal doxorubicin formulations using capillary electrophoresis.

摘要: Abstract To understand the quality, efficacy, and safety of liposomal drugs, it is necessary to develop a robust accurate method for separation quantification unencapsulated liposome-associated drugs (or encapsulated drugs). Conventional methods involve drug using solid phase extraction further quantification. This lengthy process, sometimes induces leakage from liposomes causing erroneous results. In this study, capillary electrophoresis (CE) with UV–Vis detection was developed simultaneous doxorubicin-containing liposome formulation as model drug. CE separates based on their electrophoretic mobility under electric field. Liposomal were diluted appropriate concentrations running buffer or 5% dextrose before hydrodynamic sample injection. Using high-sensitivity cell, doxorubicin sensitivity enhanced about 10-fold compared conventional on-column 75 µm i.d. column. The optimal minimal perturbation accomplished phosphate (20 mM, pH 6.5) in presence 10% sucrose.