COMBINATION OF CYTOCHEMICAL STAINING METHODS FOR ENZYME LOCALIZATION WITH ELECTRON MICROSCOPY

摘要: Publisher Summary This chapter presents a combination of cytochemical staining methods for enzyme localization with electron microscopy. It discusses problems associated the extension to Fixation not only preserves fine structure and reduces diffusion enzymes but also increases binding dyes protein, thereby ensuring better remaining activity. Osmium tetroxide fixation drastically enzymic activity gives differential inhibition in some cases. Brief these effects results superficial tissue preservation variable patterns. Formalin uniform tissues high presence calcium, is well preserved. Phosphate-buffered formol-sucrose excellent structure. The use blocks fixed nonuniform restricted outer layers cells. frozen slices formol-phosphate-sucrose-fixed show extensive areas good stain discretely throughout by esterase acid phosphatase techniques. valuable procedure microscopy as active may be stained before post-fixation osmium tetroxide, dehydration, embedding. undergo from their sites deposition during dehydration