Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cells
作者： Peter Dierks , Albert van Ooyen , Mark D. Cochran , Carl Dobkin , Jakob Reiser
DOI: 10.1016/0092-8674(83)90055-7
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摘要: Abstract Cloned rabbit β-globin genes, modified in vitro by restructuring or site-directed mutagenesis, were introduced into mouse 3T6 cells, and the resulting transcripts analyzed nuclease S1 mapping. The first 109 bp preceding cap site sufficed for maximal transcription. This segment contained three functionally important regions of ATA box region; CCAAT −100 region. latter consists an imperfect tandemly repeated sequence 14 15 bp, both copies which are required optimal promoter function. Each contains two more nucleotide positions where introduction point mutations reduces transcription at least a factor 2.
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