Suppression of the inflammatory response from adherent cells on phospholipid polymers.
作者： Shin-ichi Sawada , Shujiro Sakaki , Yasuhiko Iwasaki , Nobuo Nakabayashi , Kazuhiko Ishihara
DOI: 10.1002/JBM.A.10433
关键词:
摘要: The expression of interleukin-1beta (IL-1beta) messenger RNA (mRNA) in macrophage-like cells cultured on phospholipid polymers was evaluated to determine the extent inflammatory response. As polymers, poly(2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-butyl methacrylate(BMA)s (PMBs) were synthesized. Poly(ethylene terephthalate) (PET), poly(2-hydroxyethyl methacrylate) (PHEMA), and segmented poly(ether urethane) (Tecoflex 60) used as reference biomedical polymers. protein adsorption onto polymer surfaces from a cell culture medium determined. amount total adsorbed PMBs lower than that decreased with an increase MPC units PMBs. Human premyelocytic leukemia line (HL-60) used, IL-1beta mRNA investigated reverse transcription polymerase chain reaction (RT-PCR) method. When HL-60 PMBs, much less In particular, containing more 10 mol % not detected. This corresponded reduced proteins PMB surfaces. These results suggest effectively suppressed activation response adherent macrophagelike cells.
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