Enhanced sensitivity of LC-MS analysis of permethylated N-glycans through online purification.

作者: Janie L. Desantos-Garcia , Sarah I. Khalil , Ahmed Hussein , Yunli Hu , Yehia Mechref

DOI: 10.1002/ELPS.201100378

关键词:

摘要: … 73 N-glycan structures, while only 63 glycan structures were … aliquot of phosphate buffer saline (50 mM sodium phosphate, … 1, sialylated, high mannose, branched and hybrid N-glycans …

参考文章(28)
Yehia Mechref, Pilsoo Kang, Milos V. Novotny, Solid-phase permethylation for glycomic analysis. Methods of Molecular Biology. ,vol. 534, pp. 53- 64 ,(2009) , 10.1007/978-1-59745-022-5_4
John A. Goetz, Yehia Mechref, Pilsoo Kang, Meei-Huey Jeng, Milos V. Novotny, Glycomic profiling of invasive and non-invasive breast cancer cells. Glycoconjugate Journal. ,vol. 26, pp. 117- 131 ,(2009) , 10.1007/S10719-008-9170-4
Radoslav Goldman, Habtom W. Ressom, Rency S. Varghese, Lenka Goldman, Gregory Bascug, Christopher A. Loffredo, Mohamed Abdel-Hamid, Iman Gouda, Sameera Ezzat, Zuzana Kyselova, Yehia Mechref, Milos V. Novotny, Detection of hepatocellular carcinoma using glycomic analysis. Clinical Cancer Research. ,vol. 15, pp. 1808- 1813 ,(2009) , 10.1158/1078-0432.CCR-07-5261
Yehia Mechref, Ahmed Hussein, Slavka Bekesova, Vitara Pungpapong, Min Zhang, Lacey E. Dobrolecki, Robert J. Hickey, Zane T. Hammoud, Milos V. Novotny, Quantitative serum glycomics of esophageal adenocarcinoma and other esophageal disease onsets. Journal of Proteome Research. ,vol. 8, pp. 2656- 2666 ,(2009) , 10.1021/PR8008385
P. M. Rudd, Glycosylation and the Immune System Science. ,vol. 291, pp. 2370- 2376 ,(2001) , 10.1126/SCIENCE.291.5512.2370
Ewa Pocheć, Anna Lityńska, Angela Amoresano, Annarita Casbarra, Glycosylation profile of integrin α3β1 changes with melanoma progression Biochimica et Biophysica Acta. ,vol. 1643, pp. 113- 123 ,(2003) , 10.1016/J.BBAMCR.2003.10.004
Natasha E. Zachara, Gerald W. Hart, The emerging significance of O-GlcNAc in cellular regulation. Chemical Reviews. ,vol. 102, pp. 431- 438 ,(2002) , 10.1021/CR000406U
Pilsoo Kang, Yehia Mechref, Milos V. Novotny, High-throughput solid-phase permethylation of glycans prior to mass spectrometry. Rapid Communications in Mass Spectrometry. ,vol. 22, pp. 721- 734 ,(2008) , 10.1002/RCM.3395
Ming Lei, Milos V. Novotny, Yehia Mechref, Sequential enrichment of sulfated glycans by strong anion-exchange chromatography prior to mass spectrometric measurements. Journal of the American Society for Mass Spectrometry. ,vol. 21, pp. 348- 357 ,(2010) , 10.1016/J.JASMS.2009.09.017