Effects of phenol and natural phenolic compounds on biofilm formation by Pseudomonas aeruginosa.
作者： Sumitkumar Jagani , Rahul Chelikani , Dong-Shik Kim
DOI: 10.1080/08927010802660854
关键词:
摘要: A biofilm is formed as a result of adhesion microorganisms to various surfaces with the production extracellular polymers (polysaccharides and proteins). Biofilms cause serious problems in chemical, medical pharmaceutical industries. Recent findings indicate that some natural phenolic compounds found plants have an anti-biofouling effect on formation by Gram-negative bacteria. The activities 14 selected phenol were tested against Pseudomonas aeruginosa, using microtiter-plate. modified microtiter-plate assay was used because it enabled indirect measurement bacterial cells attached surface wells. This involved fixing film methanol, staining crystal violet dye then releasing bound 33% glacial acetic acid. optical density (OD) solution measured at 570 nm automated ICN Flow Titertek Multiscan Plus reader. Phenol except ethyl linoleate tocopherol showed significant reduction P. aeruginosa.
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