Cultura primária de fibrocondrócitos de menisco de coelho Primary culture of fibrocondrocytes from rabbit knee joint meniscus.

摘要: The present study describes a primary culture of meniscus fibrocartilaginous tissue cultivated in high density technique. Cells were isolated from menisci 120 days-old New Zealand white rabbits. Menisci finely minced , and treated with 2mg/mL collagenase DMEM (Dulbecco’s modified Eagle’s medium) containing 10% FSC (fetal calf serum) shaker for three hours at 37°C. Fibrochondrocyte cells seeded (1x10 5 /cm 2 ) T25 flasks supplemented FCS. Nearly 80% attached to the flask after culturing five days uniformly displayed as an elongated, fibroblastic-like morphology. showed confluence approximately 15 produced sufficient extracellular matrix which could be evidenced by appearance toluidine blue metachromasia. No morphological changes observed fibrochondrocytes during growing. growth rate increased 2.5 fold attained stationary phase. During seven days, newly glycosaminglycans synthesizing cells, total synthesis glycosaminglycan was high, it remained stable monolayer confluency. Ultrastructural morphology eight identical typical fibrochondrocyte vivo.