Role of vascular endothelial growth factor/vascular permeability factor in the pathogenesis of Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphomas.

摘要: Primary effusion lymphomas (PELs), which are rare associated with Kaposi's sarcoma-associated herpesvirus (or human herpesvirus-8) infection, present as malignant lymphomatous effusions in body cavities. Because PELs prefer liquid growth, we hypothesized that increased vascular permeability would be required for to form. We found the PEL cell lines BC-1, BCP-1, and BCBL-1 produce high levels of endothelial growth factor/vascular factor (VEGF/VPF). Reverse transcriptase-polymerase chain reaction analysis RNA from amplified 3 VEGF-secreted isoforms: VEGF/VPF121, VEGF/VPF145, VEGF/VPF165. Two expressed VEGF/VPF receptor Flt-1, but did not stimulate proliferation these cells. Most (13/14) control SCID/beige mice inoculated intraperitoneally cells subsequently observed or treated antibodies developed lymphoma origin prominent bloody ascites. In contrast, none (0/9) a neutralizing antihuman antibody ascites lymphoma. These results demonstrate is critical suggest stimulation may pathogenesis PELs.