Pathogenesis of Ascites Tumor Growth: Vascular Permeability Factor, Vascular Hyperpermeability, and Ascites Fluid Accumulation

摘要: Previous studies have shown that accumulation of tumor ascites fluid results in large part from increased permeability peritoneal lining vessels (Nagy et al., Cancer Res., 49: 5449-5458, 1989; Nagy 53: 2631-2643, 1993). However, the specific microvessels rendered hyperpermeable not been identified nor has basis vascular hyperpermeability established. To address these questions, TA3/St and MOT carcinomas, well-characterized transplantable murine tumors grow both solid form, were studied as model systems. Ascites cells either type injected i.p. into syngeneic A/Jax C3Heb/FeJ mice, plasma collected at intervals thereafter up to 8 28 days, respectively. Beginning several days after cell injection, small blood located tissues cavity (mesentery, wall, diaphragm) became macromolecular tracers (125I-human serum albumin, FITC-dextran, colloidal carbon, Monastral Blue B). Increased microvascular correlated with appearance factor (VPF), a cell-secreted mediator potently enhances circulating macromolecules. VPF was measured by functional bioassay sensitive immunofluorometric assay. The concentration, total VPF, volume, number, found increase parallel over time. close correlation concentration development two well-defined suggests secretion is responsible, whole or part, for initiating maintaining pattern growth.