Nanoliter high throughput quantitative PCR
作者： Tom Morrison , James Hurley , Javier Garcia , Karl Yoder , Arrin Katz
DOI: 10.1093/NAR/GKL639
关键词:
摘要: Understanding biological complexity arising from patterns of gene expression requires accurate and precise measurement of RNA levels across large numbers of genes simultaneously. Real time PCR (RT-PCR) in a microtiter plate is the preferred method for quantitative transcriptional analysis but scaling RT-PCR to higher throughputs in this fluidic format is intrinsically limited by cost and logistic considerations. Hybridization microarrays measure the transcription of many thousands of genes simultaneously yet are limited by low …
oup.com
core.ac.uk
oxfordjournals.org
paperity.org参考文章(32)
Harvey F. Lodish, Molecular Cell Biology ,(1986)
David Petersen, GVR Chandramouli, Joel Geoghegan, Joanne Hilburn, Jonathon Paarlberg, Chang Hee Kim, David Munroe, Lisa Gangi, Jing Han, Raj Puri, Lou Staudt, John Weinstein, J Carl Barrett, Jeffrey Green, Ernest S Kawasaki, Three microarray platforms: An analysis of their concordance in profiling gene expression BMC Genomics. ,vol. 6, pp. 63- 63 ,(2005) , 10.1186/1471-2164-6-63
R. J. Lipshutz, D. Morris, M. Chee, E. Hubbell, M. J. Kozal, N. Shah, N. Shen, R. Yang, S. P. A. Fodor, Using oligonucleotide probe arrays to access genetic diversity Microsystem Technology: A Powerful Tool for Biomolecular Studies. ,vol. 19, pp. 241- 254 ,(1999) , 10.1007/978-3-0348-8817-2_9
Dae-Sik Lee, Se Ho Park, Haesik Yang, Kwang-Hyo Chung, Tae Hwan Yoon, Sung-Jin Kim, Kyuwon Kim, Youn Tae Kim, Bulk-micromachined submicroliter-volume PCR chip with very rapid thermal response and low power consumptionElectronic supplementary information (ESI) available: details of numerical simulation. See http://www.rsc.org/suppdata/lc/b3/b313547k/ Lab on a Chip. ,vol. 4, pp. 401- 407 ,(2004) , 10.1039/B313547K
James F. Brown, Olga V. Kalinina, Jonathan E. Silver, Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly ,(1997)
Jian Liu, Markus Enzelberger, Stephen Quake, None, A nanoliter rotary device for polymerase chain reaction. Electrophoresis. ,vol. 23, pp. 1531- 1536 ,(2002) , 10.1002/1522-2683(200205)23:10<1531::AID-ELPS1531>3.0.CO;2-D
M. Schena, D. Shalon, R. W. Davis, P. O. Brown, Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray Science. ,vol. 270, pp. 467- 470 ,(1995) , 10.1126/SCIENCE.270.5235.467
Laura Meagher, Danuta Mahiouz, Katherine Sugars, Nigel Burrows, Paul Norris, Helen Yarwood, Michael Becker-Andre, Dorian O. Haskard, Measurement of mRNA for E-selectin, VCAM-1 and ICAM-1 by reverse transcription and the polymerase chain reaction Journal of Immunological Methods. ,vol. 175, pp. 237- 246 ,(1994) , 10.1016/0022-1759(94)90366-2
Murray O. Robinson, Melvin I. Simon, Determining transcript number using the polymerase chain reaction: Pgk-2, mP2, and PGK-2 transgene mRNA levels during spermatogenesis Nucleic Acids Research. ,vol. 19, pp. 1557- 1562 ,(1991) , 10.1093/NAR/19.7.1557
Russell Higuchi, Carita Fockler, Gavin Dollinger, Robert Watson, Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions Nature Biotechnology. ,vol. 11, pp. 1026- 1030 ,(1993) , 10.1038/NBT0993-1026