OBTENÇÃO DE PLANTAS DE LIMÃO CRAVO (Citrus limonia Osbeck) E TANGERINA CLEÓPATRA (Citrus reshni Hort.) A PARTIR DO CULTIVO DE PROTOPLASTOS DE SUSPENSÃO CELULAR
作者： Rodrigo Rocha Latado , Fernando Berlink D'utra Vaz , Augusto Tulmann Neto
DOI: 10.1590/S0103-90161999000200023
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摘要: Este trabalho descreve uma metodologia para a regeneracao de plantas tangerina 'Cleopatra' e limao 'Cravo', partir do cultivo protoplastos suspensao celular. Para tal, calos nucelares foram induzidos em meio contendo BAP cultivados sem reguladores crescimento. Protoplastos isolados suspensoes celulares gotas agarose, com densidade 2 X 105 protoplastos.ml-1. O MT, acido giberelico agua coco, foi eficiente na germinacao embrioes somaticos. Os metodos aclimatacao testados apresentaram baixa eficiencia. Como resultado final, 17 adaptadas 8 obtidas.
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