Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco
作者： Chiang-Shiong Loh , Chong-Jin Goh , Gueck-Eng Sim
DOI: 10.1007/BF00269527
关键词:
摘要: Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly protoplasts an obvious intervening callus phase. As many as 1,800 somatic 4 ml protoplast (density 2×106/ml) for 35 days. Upon transferring the embryoids to with 1 mgl−1 GA3, they into plant-lets. Rooted plantlets obtained 3 months after isolation.
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sci-hub.se 参考文章(10)
Toshiyuki Nagata, Itaru Takebe, Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta. ,vol. 92, pp. 301- 308 ,(1970) , 10.1007/BF00385097
S. Kobayashi, Uniformity of plants regenerated from orange (Citrus sinensis Osb.) protoplasts. Theoretical and Applied Genetics. ,vol. 74, pp. 10- 14 ,(1987) , 10.1007/BF00290076
Liang-cai Li, HansWilly Kohlenbach, Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L. Plant Cell Reports. ,vol. 1, pp. 209- 211 ,(1982) , 10.1007/BF00270237
Tetsushi Hidaka, Ichiro Kajiura, Plantlet differentiation from callus protoplasts induced from Citrus embryo Scientia Horticulturae. ,vol. 34, pp. 85- 92 ,(1988) , 10.1016/0304-4238(88)90078-7
Jack M. Widholm, The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells Biotechnic & Histochemistry. ,vol. 47, pp. 189- 194 ,(1972) , 10.3109/10520297209116483
J. W. Grosser, F. G. Gmitter, J. L. Chandler, Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Flying Dragon Plant Cell Reports. ,vol. 7, pp. 5- 8 ,(1988) , 10.1007/BF00272965
T. Ohgawara, S. Kobayashi, E. Ohgawara, H. Uchimiya, S. Ishii, Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theoretical and Applied Genetics. ,vol. 71, pp. 1- 4 ,(1985) , 10.1007/BF00278245
A. Vardi, P. Spiegel-Roy, E. Galun, Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species. Theoretical and Applied Genetics. ,vol. 62, pp. 171- 176 ,(1982) , 10.1007/BF00293354
Shozo Kobayashi, Isami Ikeda, Hirofumi Uchimiya, Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb. ) protoplasts Plant Cell Tissue and Organ Culture. ,vol. 4, pp. 249- 259 ,(1985) , 10.1007/BF00040199
Yasutaka Miura, Mamoru Tabata, Direct somatic embryogenesis from protoplasts of Foeniculum vulgare. Plant Cell Reports. ,vol. 5, pp. 310- 313 ,(1986) , 10.1007/BF00269830