Destabilization of IncA and IncC plasmids by SGI1 and SGI2 type Salmonella genomic islands.

摘要: Both the Salmonella genomic islands (SGI) and conjugative IncC plasmids are known to contribute substantially acquisition of resistance multiple antibiotics, in A/C group mobilize island SGI1, which also carries antibiotic genes. Plasmid pRMH760 (IncC; A/C2) was shown SGI1 variants SGI1-I, SGI1-F, SGI1-K SGI2 from enterica Escherichia coli where it integrated at preferred location, end trmE (thdF) gene. The plasmid transferred a similar frequency. However, we observed that co-transfer SGI rarer. In E. transfer, frequency transfer least 1000-fold lower when donor carried SGI1-I or SGI1-K, indicating suppresses plasmid. addition, rapidly lost both S. strains SGI1-F SGI2. loss not seen variant lacks two segments backbone. complete sequence were determined single base substitutions relative SGI1-I. IncA (A/C1) RA1 SGI2-A though there significant differences between backbones plasmids, present same cell. We conclude interactions, cooperative antagonistic, an family islands.