Interaction between Shiga Toxin and Monoclonal Antibodies: Binding Characteristics and in Vitro Neutralizing Abilities

摘要: Monoclonal antibodies (MAbs) have been employed either for diagnosis or treatment of infections caused by different pathogens. Specifically Shiga toxin-producing Escherichia coli (STEC), numerous immunoassays developed STEC diagnosis, showing variability in sensitivity and specificity when evaluated reference laboratories, no therapy vaccines are currently approved. Thus, the aim this work was characterization interaction between MAbs against Stx1 Stx2 toxins their neutralizing abilities to enable use as tools therapy. The selected clones designated 3E2 (anti-Stx1) 2E11 (anti-Stx2) were classified IgG1. recognized B subunit with an affinity constant 2.5 × 10−10 M, detected little 6.2 ng stable up 50 oC. In contrast, A Stx2, 70 oC, had a high dissociation 6.1 12.5 Stx2. Neutralization tests showed that 160 MAb inhibited 80% activity 500 µg required 60% inhibition activity. These amounts reversed 25 cytotoxicity triggered isolates. conclusion, these show suitable characteristics encourage future studies investigate protective efficacy.