In vivo and in vitro release of lysozyme from cross-linked gelatin hydrogels: a model system for the delivery of antibacterial proteins from prosthetic heart valves.

摘要: Prosthetic valve endocarditis may be reduced by the local delivery of antibacterial proteins from Dacron sewing ring a prosthetic heart valve. discs were treated with carbon dioxide gas plasma to improve hydrophilicity and thereby enabling homogeneous impregnation gelatin type B. The samples cross-linked different degrees using various amounts water-soluble carbodiimide (EDC) N-hydroxysuccinimide (NHS). Lysozyme, model protein for proteins, was loaded into (non)-cross-linked gels incorporated in Dacron, or adsorbed onto non-treated plasma-treated Dacron. vivo lysozyme release measured after subcutaneous implantation lysozyme-loaded rats. content samples, level surrounding tissue determined at explantation times (ranging 6 h up 1 week). For gels, elevated 2 days implantation. In vitro agarose medium phosphate buffer. Lysozyme buffer solution under sink conditions good agreement profiles, therefore considered describe characteristics. modelled Fick’s second law diffusion appropriate boundary conditions. this way concentration gel as function time distance obtained. presence did lead an increased uptake delayed during 30 implantation, whereas burst took place containing non-cross-linked gelatin.