Bacteriocin of a Group B Streptococcus: Partial Purification and Characterization

摘要: Abstract The production of bacteriocin-like inhibition by two 135 strains group B streptococci was demonstrated during the growth these organisms on solid nutrient media. Although a variety gram-positive susceptible, none wide range different gram-negative inhibited. The active substance produced one extracted from cultures grown media (Todd-Hewitt agar) and shown to be bactericidal in action. This bacteriocin (streptocin B1) partially purified ammonium sulfate precipitation followed gel filtration ion-exchange chromatography. Production repressed addition certain fermentable carbohydrates basic medium enhanced yeast extract. exist distinct interconvertible physical forms: unit molecular weight 10,000 an aggregate having over 200,000. inactivated proteolytic enzymes and, although labile alkali, it stable boiling mild acids.