Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products
作者： Xingliang Ma , Letian Chen , Qinlong Zhu , Yuanling Chen , Yao-Guang Liu
DOI: 10.1016/J.MOLP.2015.02.012
关键词:
摘要: The recent development of sequence-specific nuclease systems, i.e., TALENs and CRISPR/Cas9, has made genomic targeting easier in many organisms including plants (Li et al., 2012; Cong 2013; Joung Sander, Li, Shan Liang 2014; Zhang 2014). Mutations induced by CRISPR/Cas9 usually occur around the cleavage sites at three bases upstream protospacer-adjacent motif (PAM), producing insertion deletion nucleotides. For diploid organisms, such targeted mutations may happen one or both homologous chromosomes.
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