Incomplete fatty acid oxidation. The production and epimerization of 3-hydroxy fatty acids.

摘要: 3-Hydroxydicarboxylic acids are major urinary metabolites derived from fatty acid metabolism. These compounds produced the omega-oxidation of 3-hydroxy acids. The production precursor incomplete beta-oxidation in rat liver mitochondria was investigated. Independent chain length or concentration substrates, accumulation 3-hydroxyacyl intermediates relatively constant at 3-5 nmol/mg mitochondrial protein. extent oxidation same Percoll gradient-purified mitochondria. Rotenone treatment increased 3-Hydroxy did not exist as pure L-enantiomer expected beta-oxidation. Instead, these were epimerized to a near racemic mixture D- and L-isomers with slightly dominant D-isomer (58 +/- 3%). By using deuterium-isotope labeling, mechanism epimerizartion shown be rapid dehydration-rehydration through trans-2-enoyl-CoA. In addition, cis-3 trans-3 produced; isomerization Epimerase isomerase thought enzymes involved unsaturated Current data have that metabolism is actually NADPH-dependent reduction pathways. activities epimerase detected possibly function mainly saturated reverse role conventional concept.