Redox control of beta-oxidation in rat liver mitochondria.
作者： Simon EATON , Douglass M. TURNBULL , Kim BARTLETT
DOI: 10.1111/J.1432-1033.1994.TB18668.X
关键词: Respiratory chain 、 Carnitine 、 Reverse electron flow 、 Beta oxidation 、 NAD+ kinase 、 Cofactor 、 Redox 、 Palmitoyl-CoA 、 Biochemistry 、 Chemistry
摘要: Coupled rat liver mitochondria were incubated with [U-14C]hexadecanoate and carnitine which resulted in the formation of acyl-, 2-enoyl- 3-hydroxyacyl-CoA esters. The production 2-enoyl-CoA esters was associated a significant lowering NAD+/NADH ratio, contrast to muscle [Eaton, S., Bhuiyan, A. K. M. J., Kler, R. Turnbull, D. & Bartlett, (1993) Biochem. J. 289, 161-172], suggesting that control by respiratory chain is important under normal conditions. When ratios held low succinate-induced reverse electron flow, 3-enoyl-CoA also detected, probably formed action 3,2-enoyl-CoA isomerase. Measurement flux beta-oxidation at different osmolalities showed strongly dependent on osmolality changes physiological range. CoA resulting from incubations made there an increase amounts saturated acyl-CoA respect esters, consistent electron-transfer flavoprotein-ubiquinone segment [Halestrap, P. Dunlop, L. (1986) 239, 559-565]. This however could not be only factor operating as indicated continued presence high osmolalities.
sci-hub.se 参考文章(48)
A P Halestrap, J L Dunlop, Intramitochondrial regulation of fatty acid β-oxidation occurs between flavoprotein and ubiquinone A role for changes in the matrix volume Biochemical Journal. ,vol. 239, pp. 559- 565 ,(1986) , 10.1042/BJ2390559
N J Watmough, D M Turnbull, H S A Sherratt, K Bartlett, Measurement of the acyl-CoA intermediates of beta-oxidation by h.p.l.c. with on-line radiochemical and photodiode-array detection. Application to the study of [U-14C]hexadecanoate oxidation by intact rat liver mitochondria. Biochemical Journal. ,vol. 262, pp. 261- 269 ,(1989) , 10.1042/BJ2620261
K Melde, S Jackson, K Bartlett, H S A Sherratt, S Ghisla, Metabolic consequences of methylenecyclopropylglycine poisoning in rats. Biochemical Journal. ,vol. 274, pp. 395- 400 ,(1991) , 10.1042/BJ2740395
Lars Ernster, Chuan-pu Lee, [112] Energy-linked reduction of NAD+ by succinate Methods in Enzymology. ,vol. 10, pp. 729- 738 ,(1967) , 10.1016/0076-6879(67)10120-1
B Sumegi, Paul A Srere, None, Complex I binds several mitochondrial NAD-coupled dehydrogenases. Journal of Biological Chemistry. ,vol. 259, pp. 15040- 15045 ,(1984) , 10.1016/S0021-9258(17)42511-7
Y Uchida, K Izai, T Orii, T Hashimoto, Novel fatty acid beta-oxidation enzymes in rat liver mitochondria. II. Purification and properties of enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein. Journal of Biological Chemistry. ,vol. 267, pp. 1034- 1041 ,(1992) , 10.1016/S0021-9258(18)48391-3
Edith Lerner, Austin L. Shug, Charles Elson, Earl Shrago, Reversible Inhibition of Adenine Nucleotide Translocation by Long Chain Fatty Acyl Coenzyme A Esters in Liver Mitochondria of Diabetic and Hibernating Animals Journal of Biological Chemistry. ,vol. 247, pp. 1513- 1519 ,(1972) , 10.1016/S0021-9258(19)45587-7
S J Jin, C L Hoppel, K Y Tserng, Incomplete fatty acid oxidation. The production and epimerization of 3-hydroxy fatty acids. Journal of Biological Chemistry. ,vol. 267, pp. 119- 125 ,(1992) , 10.1016/S0021-9258(18)48467-0
S. Mahadevan, M. Malaiyandi, J.D. Erfle, F. Sauer, Metabolism of l-Carnitine Esters of β-Substituted Palmitic Acid by Rat Liver Mitochondria Journal of Biological Chemistry. ,vol. 245, pp. 3218- 3224 ,(1970) , 10.1016/S0021-9258(18)63043-1
J.C. Mathai, Z.E. Sauna, O. John, V. Sitaramam, Rate-limiting step in electron transport. Osmotically sensitive diffusion of quinones through voids in the bilayer. Journal of Biological Chemistry. ,vol. 268, pp. 15442- 15454 ,(1993) , 10.1016/S0021-9258(18)82277-3