Identification and Characterization of Modification Enzymes by Biochemical Analysis of the Proteome
作者： Jane E. Jackman , Lakmal Kotelawala , Elizabeth J. Grayhack , Eric M. Phizicky
DOI: 10.1016/S0076-6879(07)25006-2
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摘要: Abstract The use of proteomic libraries designed to express the complete set proteins from an organism has resulted in identification many RNA modification enzymes whose function was previously unknown. Here we describe a generalized procedure for biochemical analysis yeast library nucleic acid–modifying enzymes, by MORF (Moveable Open Reading Frame) (Gelperin et al., 2005) as source protein activity, and known tRNA methyltransferase Trm4 test case. procedures outlined this chapter can be applied any expression organism, which will become increasingly available number sequenced genomes increases genomic cloning techniques improve.
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