Protein Production in Saccharomyces cerevisiae for Systems Biology Studies
作者： Naglis Malys , Jill A. Wishart , Stephen G. Oliver , John E.G. McCarthy
DOI: 10.1016/B978-0-12-385118-5.00011-6
关键词: Saccharomyces cerevisiae 、 Systems biology 、 Protein biosynthesis 、 Context (language use) 、 Computational biology 、 A protein 、 Biology 、 Cell biology 、 Gene expression 、 Nucleic acid 、 Intracellular
摘要: Proteins together with metabolites, nucleic acids, lipids, and other intracellular molecules form biological systems that involve networks of functional physical interactions. To understand these interactions the many characteristics proteins in context biochemical biology, research aimed at studying medium large sets is required. This either involves an investigation focused on individual protein activities mixture (e.g., cell extracts) or a characterization isolated form. chapter provides overview currently available resources strategies for isolation from Saccharomyces cerevisiae. The use standardized gene expression discussed, production protocols applied to data generation pipeline biology are described detail.
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Sarah Zerbs, Ashley M. Frank, Frank R. Collart, Bacterial systems for production of heterologous proteins. Methods in Enzymology. ,vol. 463, pp. 149- 168 ,(2009) , 10.1016/S0076-6879(09)63012-3
William H. Brondyk, Selecting an appropriate method for expressing a recombinant protein. Methods in Enzymology. ,vol. 463, pp. 131- 147 ,(2009) , 10.1016/S0076-6879(09)63011-1
Jane E. Jackman, Lakmal Kotelawala, Elizabeth J. Grayhack, Eric M. Phizicky, Identification and Characterization of Modification Enzymes by Biochemical Analysis of the Proteome Methods in Enzymology. ,vol. 425, pp. 139- 152 ,(2007) , 10.1016/S0076-6879(07)25006-2
Pedro Mendes, Hanan Messiha, Naglis Malys, Stefan Hoops, Enzyme kinetics and computational modeling for systems biology. Methods in Enzymology. ,vol. 467, pp. 583- 599 ,(2009) , 10.1016/S0076-6879(09)67022-1
Kathleen M. Carroll, Francesco Lanucara, Claire E. Eyers, Quantification of Proteins and Their Modifications Using QconCAT Technology Methods in Enzymology. ,vol. 500, pp. 113- 131 ,(2011) , 10.1016/B978-0-12-385118-5.00007-4
Joseph B. Franklin, Elisabetta Ullu, Biochemical analysis of PIFTC3, the Trypanosoma brucei orthologue of nematode DYF‐13, reveals interactions with established and putative intraflagellar transport components Molecular Microbiology. ,vol. 78, pp. 173- 186 ,(2010) , 10.1111/J.1365-2958.2010.07322.X
James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden, Thomas Chappell, Expression in the yeast Pichia pastoris. Methods in Enzymology. ,vol. 463, pp. 169- 189 ,(2009) , 10.1016/S0076-6879(09)63013-5
Guillaume Rigaut, Anna Shevchenko, Berthold Rutz, Matthias Wilm, Matthias Mann, Bertrand Séraphin, None, A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology. ,vol. 17, pp. 1030- 1032 ,(1999) , 10.1038/13732
Donald L. Jarvis, Baculovirus-insect cell expression systems. Methods in Enzymology. ,vol. 463, pp. 191- 222 ,(2009) , 10.1016/S0076-6879(09)63014-7
Arun Malhotra, Tagging for protein expression Methods in Enzymology. ,vol. 463, pp. 239- 258 ,(2009) , 10.1016/S0076-6879(09)63016-0