Towards a Full Quantitative Description of Yeast Metabolism

摘要: In order to produce a full quantitative description of yeast metabolism, number kinetic parameters enzymes that are important for energy metabolism must be determined experimentally. We aim determine the prospective in vivo properties range yeast-purified isoenzymes with respect concentration their substrates and products. This endeavor forms part our systems biology pipeline facilitate production bottom-up models metabolism. Within this workflow, we implement an infrastructure medium- high-throughput determination purified like conditions. includes use KineticsWizard software data capture analysis. The captured experimental analyzed by subsequently stored appropriate repositories (MeMo-RK SABIO-RK). While focus initially on glycolysis Saccharomyces cerevisiae, methodology is generic can widely applied study other pathways organisms.