Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury
作者： Hirofumi Nishizono , Masaki Shioda , Toru Takeo , Tetsumi Irie , Naomi Nakagata
DOI: 10.1095/BIOLREPROD.103.024422
关键词:
摘要: In general, the fertilizing ability of cryopreserved mouse spermatozoa is less than that fresh spermatozoa. This especially low in C57BL/6, main strain used for production transgenic mice. To solve this problem, relationship between cell damage and was examined study. Sperm motility analysis revealed no significant difference among motilities C57BL/6J, BALB/cA, DBA/2N sperm (67.6%, 43.4%, 60.0%, respectively) after thawing. However, results vitro fertilization (IVF), scanning electron microscopy (SEM), transmission (TEM) showed a strong correlation frequency aberrant (FAS) rates (FR; C57BL/6J: FAS, 83.7%; FR, 17.0%; BALB/cA: 67.2%; 24.2%; DBA/2N: 10.2%; 93.6%), to localized particularly acrosome head mitochondria.
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