Effects of the biocide methylisothiazolinone on Xenopus laevis wound healing and tail regeneration.

摘要: The South African clawed frog, Xenopus laevis, has a strong history as suitable model for environmental studies. Its embryos and transparent tadpoles are highly sensitive to the environment their developmental processes well described. It is also amenable molecular These characteristics enable its use rapid identification understanding of exposure-induced defects. To investigate consequences chemical exposure on aquatic animals, laevis were exposed biocide, methylisothiazolinone (MIT). Frog MIT following tail amputation lost natural regenerative ability. This inhibition regeneration led failure regrow tissues including spinal cord, muscle, notochord. MIT-dependent defect due close wound. A wound healing assay revealed that while untreated wounds within one day after injury, MIT-treated animals maintained open did not reduce in size caused lethality. Concomitant with chemicals containing thiol groups such glutathione N-acetyl cysteine restored normal responses tadpoles. Together these results indicate impairs repair tissue laevis. Thus, this study reveals new aspects activity demonstrates well-suited facilitating future research into effects injury responses.