Increase in larval gut proteolytic activities and Bti resistance in the Dengue fever mosquito.

作者: Guillaume Tetreau , Renaud Stalinski , Jean-Philippe David , Laurence Després

DOI: 10.1002/ARCH.21076

关键词:

摘要: The bioinsecticide Bacillus thuringiensis var. israelensis (Bti) is increasingly used worldwide for mosquito control. Although no established resistance to Bti has been described in the field so far, a resistant Aedes aegypti strain (LiTOX strain) was selected laboratory using field-collected leaf litter containing toxins. This exhibits moderate level Bti, but high individual Cry As contains four different toxins, generalist mechanisms affecting tolerance toxins were expected strain. In present work, we show that an increase of various gut proteolytic activities including trypsins, leucine-aminopeptidases, and carboxypeptidase A activities. These elevated resulted faster activation Cry4Aa protoxins while Cry4Ba or Cry11Aa not affected. results suggest changes may contribute mosquitoes together with other mechanisms.

参考文章(46)
A Vijayalakshmi, V Girish, Affordable image analysis using NIH Image/ImageJ. Indian Journal of Cancer. ,vol. 41, pp. 47- ,(2004)
Maday Alonso-del-Rivero, Sebastian A. Trejo, Mónica Rodríguez de la Vega, Yamile González, Silvia Bronsoms, Francesc Canals, Julieta Delfín, Joaquin Diaz, Francesc X. Aviles, María A. Chávez, A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magnifica--a step into the invertebrate world of proteases. FEBS Journal. ,vol. 276, pp. 4875- 4890 ,(2009) , 10.1111/J.1742-4658.2009.07187.X
Lianet Rodríguez–Cabrera, Damian Trujillo–Bacallao, Orlando Borrás–Hidalgo, Denis J Wright, Camilo Ayra–Pardo, None, RNAi-mediated knockdown of a Spodoptera frugiperda trypsin-like serine-protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca1 protoxin Environmental Microbiology. ,vol. 12, pp. 2894- 2903 ,(2010) , 10.1111/J.1462-2920.2010.02259.X
VANDANA K. HIVRALE, PURUSHOTTAM R. LOMATE, NEETA D. KALVE, MANVENDRA S. KACHOLE, Periplaneta americana midgut proteases differentially expressed against dietary components from different plant seeds Physiological Entomology. ,vol. 36, pp. 180- 186 ,(2011) , 10.1111/J.1365-3032.2011.00784.X
Ranad Shaheen, Birgitta Svensson, Maria A. Andersson, Anders Christiansson, Mirja Salkinoja-Salonen, Persistence strategies of Bacillus cereus spores isolated from dairy silo tanks Food Microbiology. ,vol. 27, pp. 347- 355 ,(2010) , 10.1016/J.FM.2009.11.004
Carmen Forcada, Encarna Alcácer, M. Dolores Garcerá, Rafael Martínez, DIFFERENCES IN THE MIDGUT PROTEOLYTIC ACTIVITY OF TWO HELIOTHIS VIRESCENS STRAINS, ONE SUSCEPTIBLE AND ONE RESISTANT TO BACILLUS THURINGIENSIS TOXINS Archives of Insect Biochemistry and Physiology. ,vol. 31, pp. 257- 272 ,(1996) , 10.1002/(SICI)1520-6327(1996)31:3<257::AID-ARCH2>3.0.CO;2-V
David G. Heckel, Linda J. Gahan, Simon W. Baxter, Jian-Zhou Zhao, Anthony M. Shelton, Fred Gould, Bruce E. Tabashnik, The diversity of Bt resistance genes in species of Lepidoptera Journal of Invertebrate Pathology. ,vol. 95, pp. 192- 197 ,(2007) , 10.1016/J.JIP.2007.03.008
Rajko Igić, Sandra Garber, Marin Sekosan, Renata A Urbanska, Rahim Behnia, Localization of carboxypeptidase A-like enzyme in rat kidney. Peptides. ,vol. 24, pp. 1237- 1240 ,(2003) , 10.1016/J.PEPTIDES.2003.07.007
Vahid Hosseininaveh, Alireza Bandani, Parvaneh Azmayeshfard, Saman Hosseinkhani, Majid Kazzazi, None, Digestive proteolytic and amylolytic activities in Trogoderma granarium Everts (Dermestidae: Coleoptera) Journal of Stored Products Research. ,vol. 43, pp. 515- 522 ,(2007) , 10.1016/J.JSPR.2007.02.003