The genetic architecture of a complex trait: Resistance to multiple toxins produced by Bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito Aedes aegypti

摘要: The bacterial insecticide Bacillus thuringiensis subsp. israelensis (Bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. Because Bti toxicity relies on the action of four main toxins, resistance very likely a complex phenotype involving several genes simultaneously. Dissecting underlying genetic basis thus requires associating quantitative measure variation at many loci in segregating population. Here, we undertake this task using dengue and yellow fever vector, Aedes aegypti, as study model. We conducted QTL (Quantitative Trait Locus) admixture mapping analyses two controlled crosses artificial admixed population, respectively, all obtained from resistant susceptible lab strains. detected 16 regions, among which QTLs were revealed by different analysis methods. These robust explained altogether 29.2% 62.2% total phenotypic variance crosses, respectively. They also showed dominant mode action. In addition, found six showing statistical association with Five supercontigs highlighted contained candidate suggested their function, or prior evidence expression and/or outlier analyses. genomic regions are good starting points fine functional aiming identifying mutations. Moreover, purpose work, built first Ae. aegypti map based markers associated expressed larvae. This harbors 229 SNP mapped across three chromosomes length 311.9cM. It brought light assembly discrepancies reference genome, suggesting high level genome plasticity aegypti.