Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line.
作者： Y Shaul , M Ziemer , P D Garcia , R Crawford , H Hsu
DOI: 10.1128/JVI.51.3.776-787.1984
关键词:
摘要: We report here the isolation by molecular cloning and analysis heteroduplex restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were present. Three different patterns organization detected. These included two linear without detectable rearrangement, three other with internal deletions, long inverted duplications. HBsAg are preferentially in integrated fragment, whereas core gene is eliminated. Several might act as templates for synthesis functional mRNA, only one clone could produce a full antigen transcript.
unab.cl
sci-hub.st 参考文章(26)
W Szmuness, Hepatocellular carcinoma and the hepatitis B virus: evidence for a causal association. Progress in medical virology. Fortschritte der medizinischen Virusforschung. Progres en virologie medicale. ,vol. 24, pp. 40- 69 ,(1978)
C Pourcel, A Louise, M Gervais, N Chenciner, M F Dubois, P Tiollais, Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA. Journal of Virology. ,vol. 42, pp. 100- 105 ,(1982) , 10.1128/JVI.42.1.100-105.1982
O Laub, L B Rall, M Truett, Y Shaul, D N Standring, P Valenzuela, W J Rutter, Synthesis of hepatitis B surface antigen in mammalian cells: Expression of the entire gene and the coding region Journal of Virology. ,vol. 48, pp. 271- 280 ,(1983) , 10.1128/JVI.48.1.271-280.1983
K. Takahashi, M. Imai, M. Mayumi, T. Mishiro, Y. Akahane, T. Gotanda, Y. Miyakawa, Demonstration of Hepatitis B e Antigen in the Core of Dane Particles Journal of Immunology. ,vol. 122, pp. 275- 279 ,(1979)
W. Kam, L. B. Rall, E. A. Smuckler, R. Schmid, W. J. Rutter, Hepatitis B viral DNA in liver and serum of asymptomatic carriers. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 7522- 7526 ,(1982) , 10.1073/PNAS.79.23.7522
G M MacNab, J J Alexander, G Lecatsas, E M Bey, J M Urbanowicz, Hepatitis B surface antigen produced by a human hepatoma cell line British Journal of Cancer. ,vol. 34, pp. 509- 515 ,(1976) , 10.1038/BJC.1976.205
Roberto Cattaneo, Hans Will, Nouria Hernandez, Heinz Schaller, Signals regulating hepatitis B surface antigen transcription Nature. ,vol. 305, pp. 336- 338 ,(1983) , 10.1038/305336A0
Jeffrey C. Edman, Patrick Gray, Pablo Valenzuela, Leslie B. Rall, William J. Rutter, Integration of hepatitis B virus sequences and their expression in a human hepatoma cell Nature. ,vol. 286, pp. 535- 538 ,(1980) , 10.1038/286535A0
F. Blattner, A. Blechl, K Denniston-Thompson, H. Faber, J. Richards, J. Slightom, P. Tucker, O Smithies, Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections. Science. ,vol. 202, pp. 1279- 1284 ,(1978) , 10.1126/SCIENCE.725603
Catherine M. Houck, Frank P. Rinehart, Carl W. Schmid, A ubiquitous family of repeated DNA sequences in the human genome. Journal of Molecular Biology. ,vol. 132, pp. 289- 306 ,(1979) , 10.1016/0022-2836(79)90261-4