Biochemical detection of hepatitis B virus constituents.
作者： Hsiang Ju Lin
DOI: 10.1016/S0065-2423(08)60183-3
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摘要: Publisher Summary This chapter discusses biochemical methods for the detection of hepatitis B virus (HBV) constituents. Hepatitis is smallest DNA viruses that infect man. Only one other animal infects pigs smaller. The four viruses—HBV, woodchuck virus, ground squirrel and duck virus—form a distinct group reverse transcribing show strong similarities in virion size, length genome, properties. Two techniques are applicable to HBV DNA: dot hybridization provides semiquantitative test its presence or absence, southern blot analysis, by means which sequences located nucleic acid fragments separated according electrophoretic mobility. principal tool studying molecular forms technique. size secondary tertiary structure gel electrophoresis transferred from membrane either blotting electrophoresis.
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