Imaging of single human carcinoma cells in vitro using a clinical whole-body magnetic resonance scanner at 3.0 T.
作者： Jens Pinkernelle , Ulf Teichgräber , Fabian Neumann , Lukas Lehmkuhl , Jens Ricke
DOI: 10.1002/MRM.20455
关键词:
摘要: The purpose of the present study was to examine whether single human carcinoma cells labeled with iron oxide nanoparticles could be detected by magnetic resonance (MR) imaging on a clinical 3-T scanner using surface coil only. WiDr colon were loaded two kinds differing coating and size: aminosilan-coated (MagForce) carboxy-dextran-coated particles (Resovist). latter preferred cell line used here taken up much faster (12 h) than smaller carboxydextran-coated Resovist (48 h). Labeled cells, distributed in an agarose gel monodisperse layer as controlled light microscopy, became detectable punctuate signal extinctions when small circularly polarized conjunction T2*-weighted GE sequence at 3 T. threshold for detectability ranged load 4 –5 g iron/10 6 cells. Obviating need special hardware additions, this opens new lane single-cell tracking MR scanners amenable patient studies. Magn Reson Med 53:1187–1192, 2005. © 2005 Wiley-Liss, Inc.
sci-hub.se 参考文章(19)
Peter Reimer, Thomas Balzer, Ferucarbotran (Resovist): a new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver: properties, clinical development, and applications. European Radiology. ,vol. 13, pp. 1266- 1276 ,(2003) , 10.1007/S00330-002-1721-7
Kathleen A. Hinds, Jonathan M. Hill, Erik M. Shapiro, Mikko O. Laukkanen, Alfonso C. Silva, Christian A. Combs, Timothy R. Varney, Robert S. Balaban, Alan P. Koretsky, Cynthia E. Dunbar, Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood. ,vol. 102, pp. 867- 872 ,(2003) , 10.1182/BLOOD-2002-12-3669
James B Aguayo, Stephen J Blackband, Joseph Schoeniger, Mark A Mattingly, Markus Hintermann, None, Nuclear magnetic resonance imaging of a single cell Nature. ,vol. 322, pp. 190- 191 ,(1986) , 10.1038/322190A0
Christopher H. Dodd, Hui-Chen Hsu, Wen-Jang Chu, Pingar Yang, Huang-Ge Zhang, John D. Mountz, Kurt Zinn, John Forder, Lee Josephson, Ralph Weissleder, James M. Mountz, John D. Mountz, Normal T-cell response and in vivo magnetic resonance imaging of T cells loaded with HIV transactivator-peptide-derived superparamagnetic nanoparticles. Journal of Immunological Methods. ,vol. 256, pp. 89- 105 ,(2001) , 10.1016/S0022-1759(01)00433-1
Anna Moore, Phillip Zhe Sun, David Cory, Dagmar Högemann, Ralph Weissleder, Myra A. Lipes, MRI of insulitis in autoimmune diabetes. Magnetic Resonance in Medicine. ,vol. 47, pp. 751- 758 ,(2002) , 10.1002/MRM.10110
Paula Foster-Gareau, Chris Heyn, Andrew Alejski, Brian K. Rutt, Imaging single mammalian cells with a 1.5 T clinical MRI scanner. Magnetic Resonance in Medicine. ,vol. 49, pp. 968- 971 ,(2003) , 10.1002/MRM.10417
Christina L. Kaufman, Mangay Williams, L. Madison Ryle, Traci L. Smith, Mike Tanner, Chien Ho, Superparamagnetic iron oxide particles transactivator protein-fluorescein isothiocyanate particle labeling for in vivo magnetic resonance imaging detection of cell migration: uptake and durability. Transplantation. ,vol. 76, pp. 1043- 1046 ,(2003) , 10.1097/01.TP.0000090164.42732.47
Yuqing Zhang, Stephen J. Dodd, Kristy S. Hendrich, Mangay Williams, Chien Ho, Magnetic resonance imaging detection of rat renal transplant rejection by monitoring macrophage infiltration Kidney International. ,vol. 58, pp. 1300- 1310 ,(2000) , 10.1046/J.1523-1755.2000.00286.X
L. Ciobanu, C.H. Pennington, 3D micron-scale MRI of single biological cells Solid State Nuclear Magnetic Resonance. ,vol. 25, pp. 138- 141 ,(2004) , 10.1016/J.SSNMR.2003.03.008
K Shah, A Jacobs, X O Breakefield, R Weissleder, Molecular imaging of gene therapy for cancer. Gene Therapy. ,vol. 11, pp. 1175- 1187 ,(2004) , 10.1038/SJ.GT.3302278