Comprehensive untargeted lipidomic analysis using core–shell C30 particle column and high field orbitrap mass spectrometer

摘要: The goal of untargeted lipidomics is to have high throughput, yet comprehensive and unambiguous identification quantification lipids. Novel stationary phases in LC separation new mass spectrometric instruments capable resolving power faster scanning rate are essential achieving this goal. In work, 4 reversed phase columns coupled with a field quadrupole orbitrap spectrometer (Q Exactive HF) were thoroughly compared using complex lipid standard mixture rat plasma liver samples. A good all lipids was achieved 24min gradient. include C30 C18 functionalization on either core-shell or totally porous silica particles, size ranging from 1.7 2.6μm. Accucore column showed the narrowest peaks highest theoretical plate number, excellent peak capacity retention time reproducibility (<1% deviation). As result, it resulted 430 species identified samples confidence. resolution offered by up-front RPLC allowed discrimination cis/trans isomeric species, afforded clear distinction isobaric full scan MS assignment sn-positional isomers for lysophospholipids MS/MS. Taken together, efficiency analysis very promising tools analysis.