作者: B. E. Branham , R. D. Plattner
DOI: 10.1007/BF01103109
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摘要: The biosynthesis of fumonisin B1 byFusarium moniliforme was studied in liquid culture. Stable isotope labeled alanine added as a precursor to static and shaken cultures ofF. moniliforme. Incorporation of13C and2H L-alanine into measured by GC-MS. Under culture conditions, 20.8 µg ml−1 FB1 were produced with 5.5% level incorporation intact, B1. shake much higher levels reaching 159–240 21 days after initiation. A lower incorporation, from 1.1–1.4%, observed under these conditions. reduced because the rapid metabolism combined high production resulted turnover added, percentage incorporation. evidence presented indicates that is incorporated intact