Effect of biostimulants on 2,4,6-trinitrotoluene (TNT) degradation and bacterial community composition in contaminated aquifer sediment enrichments.

摘要: 2,4,6-Trinitrotoluene (TNT) is a toxic and persistent explosive compound occurring as contaminant at numerous sites worldwide. Knowledge of the microbial dynamics driving TNT biodegradation limited, particularly in native aquifer sediments where it poses threat to water resources. The purpose this study was quantify effect organic amendments on anaerobic rate pathway an enrichment culture obtained from historically contaminated sediment compare bacterial community dynamics. readily biodegraded all microcosms, with highest under lactate amended condition followed by ethanol naturally matter (extracted site sediment) conditions. Although reductive degradation observed across conditions, denaturing gradient gel electrophoresis (DGGE) analysis revealed distinct compositions. In Gram-negative γ- or β-Proteobacteria Gram-positive Negativicutes Clostridia were observed. A Pseudomonas sp. particular be stimulated According non-metric multidimensional scaling DGGE profiles, microcosm communities most similar heavily TNT-contaminated field sediment, relative moderately uncontaminated sediments, suggesting that contamination itself major driver structure. Overall these results provide new line evidence key bacteria their response carbon amendment, supporting approach promising technology for stimulating situ bioremediation subsurface.