Enhanced Antigen Transport Across Rat Tracheal Epithelium Induced by Sensitization and Mast Cell Activation

摘要: Ag challenge to the apical surface of tracheal epithelium results in a rapid ion secretory response due activation mast cells. The aim this study was examine impact sensitization and specific on timing, route, quantity transported across epithelium. After rats model protein, HRP, tissues were excised mounted Ussing chambers. Tracheas from HRP-sensitized rats, but not naive or OVA-sensitized responded HRP with rise short-circuit current (beginning at approximately 2 min). Photomicrographs fixed min showed that initial transepithelial transport occurred via endosomes significantly enhanced compared both control groups. In addition, nonciliated cells, proportion which increased after sensitization, contained more than ciliated hypersensitivity only challenged associated conductance overall flux tissue. This elevated observed cell-deficient Ws/Ws rats. 90 also paracellular spaces between adjacent epithelial We conclude increases uptake initially an endosomal transcellular pathway that, reaction, cell-dependent recruitment further augments influx into airway